Whole-genome or shotgun metagenomics involves sequencing all the DNA in a sample to reconstruct the composition of the communities present and determine the functions performed by these communities (i.e. who is there and what are they doing …).
Similarly, metatranscriptomics involves studying the totality of RNAs produced by the transcription of the genomes of all organisms within a given environment.
Services
Whole-genome metagenomics and metatranscriptomics are available as short-read sequencing on the Illumina NextSeq 2000 sequencer and as long-read sequencing on the Oxford Nanopore Technologies P2 Solo sequencer.
Requirements
A minimum of 1 µg of high-molecular-weight DNA (as determined by fluorescence) is required for whole-genome metagenomics on an Illumina sequencer, and a minimum of 2 µg for whole-genome metagenomics on an Oxford Nanopore sequencer. Further details can be found in the WGS requirements.
Results
Sent by email or uploaded to a server
- Demultiplexed FASTQ files
- Sequencing run report (MultiQC)
- Analysis using Dragen Metagenomicfor Illumina or WIMP for Oxford Nanopore