Unlike DNA sequencing, which can detect the presence of variants (SNPs, INDELs, SSRs or microsatellites, etc.) without prior knowledge, genotyping focuses on known polymorphisms at specific locations in the genome.
PGTB offers a range of low- and medium-throughput genotyping solutions, which can be tailored to the type and number of markers targeted, as well as the number of samples to be analysed.
SSR genotyping or SSRseq, is both a genotyping technique—as it targets pre-identified SSRs—and a sequencing technique, as it enables the detection of new polymorphisms in the flanking regions of the targeted SSR. This approach is particularly well-suited to population genetics studies, kinship analysis, or for densifying genetic maps. Several hundred samples can be analysed simultaneously across dozens of markers. The PGTB also offers a comprehensive solution for orphan species lacking genomic resources.
SNP genotyping or SNPseq, is based on the same principle as SSRseq but targets several dozen markers across large sample sets (ranging from several hundred to several thousand). Here too, PGTB offers a comprehensive solution, right through to the production of genotype tables.