The PGTB offers a method for SNP genotyping using high-throughput sequencing which has several advantages:
– the ability to analyse up to 192 markers in a single multiplex
– the ability to genotype between 95 and 1,140 samples in a single run
- the identification of all types of polymorphisms present in the region surrounding the SNP of interest.
Services
We offer a full service comprising:
- the design of multiplex primers based on the SNP marker sequence file
- their amplification using highly multiplexed PCR (up to 192 markers tested)
- the construction of libraries and Illumina sequencing (up to 1,140 individuals per run, including 95 repeat samples for data validation during the initial analysis used to develop the markers)
- the bioinformatics analysis used to generate the genotype table.
These steps can be adapted to suit specific requirements (number of individuals and markers to be analysed, etc.).
Requirements
Results
Sent by email or uploaded to a server.
The sequencing FASTQ files and the analysis results sent in Excel format, including:
- the coverage of each locus
- allelic information
- the genotype table