{"id":807,"date":"2022-03-01T17:35:04","date_gmt":"2022-03-01T16:35:04","guid":{"rendered":"https:\/\/pgtb.fr\/?page_id=807"},"modified":"2024-03-13T11:37:00","modified_gmt":"2024-03-13T10:37:00","slug":"sites-methyles","status":"publish","type":"page","link":"https:\/\/pgtb.fr\/en\/sites-methyles\/","title":{"rendered":"Epig\u00e9n\u00e9tique cibl\u00e9e"},"content":{"rendered":"<p class=\"has-text-align-justify wp-block-paragraph\">Cette application n&rsquo;est pour l&rsquo;instant accessible que dans le cadre de projets R&amp;D.<\/p>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">La PGTB propose une m\u00e9thode de caract\u00e9risation des sites m\u00e9thyl\u00e9s (CpG) par analyse sur syst\u00e8me MassArray d\u2019Agena Bioscience. Les cytosines m\u00e9thyl\u00e9es sont mises en d\u00e9tect\u00e9es par leur masse plus \u00e9lev\u00e9e, mise en \u00e9vidence apr\u00e8s traitement bisulfite et transcription <em>in vitro<\/em> :<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img data-recalc-dims=\"1\" fetchpriority=\"high\" decoding=\"async\" width=\"750\" height=\"397\" data-attachment-id=\"1010\" data-permalink=\"https:\/\/pgtb.fr\/en\/epityper1-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/03\/epityper1-2.png?fit=1463%2C775&amp;ssl=1\" data-orig-size=\"1463,775\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"epityper1-2\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/03\/epityper1-2.png?fit=750%2C397&amp;ssl=1\" src=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/03\/epityper1-2.png?resize=750%2C397&#038;ssl=1\" alt=\"\" class=\"wp-image-1010\" \/><\/figure>\n<\/div>\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" style=\"font-size:29px\">Services<\/h2>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">Chaque puce comporte 96 positions qui peuvent \u00eatre exploit\u00e9es selon diff\u00e9rents formats&nbsp;:<\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>96 \u00e9chantillons sur 1 cible<\/li><li>48 \u00e9chantillons sur 2 cibles<\/li><li>32 \u00e9chantillons sur 3 cibles<\/li><li>24 \u00e9chantillons sur 4 cibles<\/li><li>16 \u00e9chantillons sur 6 cibles<\/li><\/ul>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">Analyse des donn\u00e9es par le personnel de la PGTB<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Pr\u00e9requis<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/pgtb.fr\/wp-content\/uploads\/2022\/02\/i-prerequis-pour-le-genotypage-massarray-96.pdf\">Pr\u00e9requis pour le g\u00e9notypage MassArray 96<\/a><\/p>\n\n\n\n<ul class=\"wp-block-list\"><li>30 \u00b5L d\u2019ADN dilu\u00e9s \u00e0 50 ng\/\u00b5L en plaques 96 compatibles (dosage par fluorescence)<\/li><li>Noms et s\u00e9quences cibles o\u00f9 sont localis\u00e9es les CpG (taille max&nbsp;: 600pb) au format .txt ou .csv<\/li><\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">R\u00e9sultats<\/h2>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">R\u00e9sultats transmis par mail ou d\u00e9pos\u00e9s sur serveur<\/p>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">Le logiciel fournit pour chaque amplicon un tableau avec les niveaux de m\u00e9thylation de chaque CpG, la s\u00e9quence nucl\u00e9otidique associ\u00e9e, ainsi qu\u2019un \u00e9pigramme&nbsp;:<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img data-recalc-dims=\"1\" decoding=\"async\" width=\"750\" height=\"854\" data-attachment-id=\"1011\" data-permalink=\"https:\/\/pgtb.fr\/en\/epityper2-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/03\/epityper2-2.png?fit=753%2C857&amp;ssl=1\" data-orig-size=\"753,857\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"epityper2-2\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/03\/epityper2-2.png?fit=750%2C854&amp;ssl=1\" src=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/03\/epityper2-2.png?resize=750%2C854&#038;ssl=1\" alt=\"\" class=\"wp-image-1011\" \/><\/figure>\n<\/div>","protected":false},"excerpt":{"rendered":"<p>Cette application n&rsquo;est pour l&rsquo;instant accessible que dans le cadre de projets R&amp;D. La PGTB propose une m\u00e9thode de caract\u00e9risation des sites m\u00e9thyl\u00e9s (CpG) par analyse sur syst\u00e8me MassArray d\u2019Agena Bioscience. Les cytosines m\u00e9thyl\u00e9es sont mises en d\u00e9tect\u00e9es par leur masse plus \u00e9lev\u00e9e, mise en \u00e9vidence apr\u00e8s traitement bisulfite et transcription in vitro : Services<a class=\"more-link\" href=\"https:\/\/pgtb.fr\/en\/sites-methyles\/\">Continue reading <span class=\"screen-reader-text\">\u00ab\u00a0Epig\u00e9n\u00e9tique cibl\u00e9e\u00a0\u00bb<\/span><\/a><\/p>","protected":false},"author":216247060,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_crdt_document":"","advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"class_list":["post-807","page","type-page","status-publish","hentry","entry"],"jetpack_likes_enabled":true,"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/PdFmHm-d1","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/807","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/users\/216247060"}],"replies":[{"embeddable":true,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/comments?post=807"}],"version-history":[{"count":12,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/807\/revisions"}],"predecessor-version":[{"id":1943,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/807\/revisions\/1943"}],"wp:attachment":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/media?parent=807"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}