{"id":441,"date":"2022-02-04T17:45:22","date_gmt":"2022-02-04T16:45:22","guid":{"rendered":"https:\/\/pgtb.fr\/?page_id=441"},"modified":"2026-09-10T09:50:30","modified_gmt":"2026-09-10T07:50:30","slug":"developpement-et-genotypage-de-microsatellites-par-sequencage-ssr-seq-2","status":"publish","type":"page","link":"https:\/\/pgtb.fr\/en\/developpement-et-genotypage-de-microsatellites-par-sequencage-ssr-seq-2\/","title":{"rendered":"Development and genotyping of microsatellites via sequencing (SSRseq)"},"content":{"rendered":"

Microsatellites are highly polymorphic multi-allelic markers<\/strong> consisting of a sequence of a repeated motif, the number of repetitions of which varies between alleles.<\/p>\n\n\n\n

The PGTB has developed a method for microsatellite genotyping using high-throughput sequencing<\/strong> which offers two major advantages:<\/p>\n\n\n\n

(1) to identify all types of polymorphisms present in the repeat motif and in the surrounding region<\/p>\n\n\n\n

(2) be based on the DNA sequence of the alleles, thereby enabling the nature of the alleles to be definitively determined and facilitating the sharing of genotype data between projects or laboratories.<\/p>\n\n\n

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You can find more details about this method developed by the PGTB in the publication <\/a>and the poster <\/a>and the list of species for which we have developed markers on this dataverse <\/a>updated regularly.<\/p>\n\n\n

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Services<\/h2>\n\n\n\n

We offer a full service comprising:<\/p>\n\n\n\n