{"id":432,"date":"2022-02-04T17:34:55","date_gmt":"2022-02-04T16:34:55","guid":{"rendered":"https:\/\/pgtb.fr\/?page_id=432"},"modified":"2026-01-08T10:12:40","modified_gmt":"2026-01-08T09:12:40","slug":"genotypage-de-snp-et-indel-2","status":"publish","type":"page","link":"https:\/\/pgtb.fr\/en\/genotypage-de-snp-et-indel-2\/","title":{"rendered":"G\u00e9notypage de SNP par spectrom\u00e9trie de masse"},"content":{"rendered":"<p class=\"has-text-align-justify wp-block-paragraph\">La technologie iPLEX Gold sur <strong>MassARRAY System d&rsquo;Agena Bioscience<\/strong> permet l\u2019analyse de SNP di, tri et t\u00e9tra-all\u00e8liques et l&rsquo;analyse d&rsquo;INDEL de 40 pb maximum, avec un niveau de multiplexage jusqu&rsquo;\u00e0 40 marqueurs pour un m\u00eame \u00e9chantillon.<\/p>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">La m\u00e9thode consiste en la r\u00e9alisation d\u2019une premi\u00e8re PCR locus sp\u00e9cifique, suivi d\u2019une extension d\u2019amorce avec une base modifi\u00e9e (ddNTP) qui va venir s\u2019hybrider directement sur le polymorphisme d\u2019int\u00e9r\u00eat. En utilisant le spectrom\u00e8tre de masse MALDI-TOF, la masse des diff\u00e9rentes amorces d\u2019extension (comprenant la base modifi\u00e9e) va permettre d\u2019identifier les diff\u00e9rents all\u00e8les. Cette technologie tr\u00e8s robuste permet notamment l&rsquo;analyse d&rsquo;ADN d\u00e9grad\u00e9s ou en faible quantit\u00e9.<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img data-recalc-dims=\"1\" fetchpriority=\"high\" decoding=\"async\" width=\"666\" height=\"707\" data-attachment-id=\"1337\" data-permalink=\"https:\/\/pgtb.fr\/en\/massarray-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/04\/massarray.png?fit=666%2C707&amp;ssl=1\" data-orig-size=\"666,707\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"massarray\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/04\/massarray.png?fit=666%2C707&amp;ssl=1\" src=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/04\/massarray.png?resize=666%2C707&#038;ssl=1\" alt=\"\" class=\"wp-image-1337\"\/><\/figure>\n<\/div>\n\n\n<h2 class=\"wp-block-heading\" id=\"services\">Services<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Diff\u00e9rentes combinaisons d&rsquo;analyse sont possibles pour une m\u00eame puce :<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>380 \u00e9chantillons sur 1 multiplex<\/li>\n\n\n\n<li>190 \u00e9chantillons sur 2 multiplex<\/li>\n\n\n\n<li>95 \u00e9chantillons sur 4 multiplex<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Analyse des donn\u00e9es par la PGTB ou par l&rsquo;utilisateur dans nos locaux apr\u00e8s formation \u00e0 l&rsquo;utilisation du logiciel.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"prerequis\">Pr\u00e9requis<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Noms et s\u00e9quences des marqueurs SNP et INDEL au format txt pour le design des multiplex. Plus il y a de candidats, plus le design sera facilit\u00e9 et le niveau de multiplexage \u00e9lev\u00e9.<\/li>\n\n\n\n<li>30 \u00b5L d\u2019ADN dilu\u00e9s \u00e0 ~ 10 ng\/\u00b5L en plaques 96 compatibles<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">Veuillez t\u00e9l\u00e9charger les pr\u00e9requis sp\u00e9cifiques :<\/p>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-c205913f-f6a7-415b-aa4a-62106943a33d\" href=\"https:\/\/pgtb.fr\/wp-content\/uploads\/2024\/01\/i-prerequis-pour-le-genotypage-massarray-384.pdf\">I-Pr\u00e9requis pour le g\u00e9notypage MassARRAY 384<\/a><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"resultats\">R\u00e9sultats<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">R\u00e9sultats transmis par mail, d\u00e9pos\u00e9s sur serveur ou \u00e0 venir analyser dans nos locaux.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Selon le service demand\u00e9 :<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>R\u00e9sultats bruts au format xml<\/li>\n\n\n\n<li>Tableaux de g\u00e9notypes et repr\u00e9sentation graphique des g\u00e9notypes de chaque marqueur, apr\u00e8s analyse des donn\u00e9es par le personnel de la plateforme<\/li>\n<\/ul>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img data-recalc-dims=\"1\" decoding=\"async\" width=\"455\" height=\"420\" data-attachment-id=\"646\" data-permalink=\"https:\/\/pgtb.fr\/en\/sequenom-2\/\" data-orig-file=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/02\/sequenom.png?fit=455%2C420&amp;ssl=1\" data-orig-size=\"455,420\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"sequenom\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/02\/sequenom.png?fit=455%2C420&amp;ssl=1\" src=\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/02\/sequenom.png?resize=455%2C420&#038;ssl=1\" alt=\"\" class=\"wp-image-646\"\/><\/figure>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"resultats\">Publications associ\u00e9es<\/h2>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">Kremer, A.,&nbsp;<strong>Delcamp, A<\/strong>., Lesur, I., Wagner, S., Rellstab, C.,&nbsp;<strong>Guichoux, E<\/strong>., and Leroy, T<em>.<\/em>, 2024. Whole-genome screening for near-diagnostic genetic markers for four western European white oak species identification.&nbsp;Annals of Forest Science.&nbsp;<a href=\"https:\/\/doi.org\/10.1186\/s13595-024-01236-9\">https:\/\/doi.org\/10.1186\/s13595-024-01236-9<\/a><\/p>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">Capo, L.F., Degen, B., Blanc-Jolivet, C., Tysklind, N., Cavers, S., Mader, M., Meyer-Sand, B.R., Paredes-Villanueva, K., Honorio Conorado, E.N., Garcia-Davila, C.R., Troispoux, V.,&nbsp;<strong>Delcamp, A<\/strong>., &amp; Sebbenn, A.M., 2024. Timber Tracking of Jacaranda copaia from the Amazon Forest Using DNA Fingerprinting.&nbsp;<em>Forests<\/em>.<a href=\"https:\/\/doi.org\/10.3390\/f15081478\">&nbsp;https:\/\/doi.org\/10.3390\/f15081478<\/a><\/p>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">Padr\u00f3n, M., Milhes, M.,&nbsp;<strong>Delcamp, A., Guichoux, E.,<\/strong>&nbsp;Guizien, K., 2023. Development of a set of SNP markers for population genetics of the sea peach (Halocynthia papillosa). Conservation Genet Resour 15, 145\u2013147.&nbsp;<a href=\"https:\/\/doi.org\/10.1007\/s12686-023-01313-7\">https:\/\/doi.org\/10.1007\/s12686-023-01313-7<\/a><\/p>\n\n\n\n<p class=\"has-text-align-justify wp-block-paragraph\">Blanc-Jolivet C, Mader M, Bouda H-N,&nbsp;<strong>Massot M<\/strong>, Da\u00efnou K, Yene G, et al. Development of new SNP and INDEL loci for the valuable African timber species Lophira alata. Conservation Genet Resour. mars 2021;13(1):85\u20117.&nbsp;<a href=\"https:\/\/doi.org\/10.1007\/s12686-020-01173-5\" target=\"_blank\" rel=\"noreferrer noopener\">https:\/\/doi.org\/10.1007\/s12686-020-01173-5<\/a><\/p>","protected":false},"excerpt":{"rendered":"<p>La technologie iPLEX Gold sur MassARRAY System d&rsquo;Agena Bioscience permet l\u2019analyse de SNP di, tri et t\u00e9tra-all\u00e8liques et l&rsquo;analyse d&rsquo;INDEL de 40 pb maximum, avec un niveau de multiplexage jusqu&rsquo;\u00e0 40 marqueurs pour un m\u00eame \u00e9chantillon. La m\u00e9thode consiste en la r\u00e9alisation d\u2019une premi\u00e8re PCR locus sp\u00e9cifique, suivi d\u2019une extension d\u2019amorce avec une base modifi\u00e9e<a class=\"more-link\" href=\"https:\/\/pgtb.fr\/en\/genotypage-de-snp-et-indel-2\/\">Continue reading <span class=\"screen-reader-text\">\u00ab\u00a0G\u00e9notypage de SNP par spectrom\u00e9trie de masse\u00a0\u00bb<\/span><\/a><\/p>","protected":false},"author":216247060,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_crdt_document":"","advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"footnotes":""},"class_list":["post-432","page","type-page","status-publish","hentry","entry"],"jetpack_likes_enabled":true,"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/PdFmHm-6Y","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/432","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/users\/216247060"}],"replies":[{"embeddable":true,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/comments?post=432"}],"version-history":[{"count":30,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/432\/revisions"}],"predecessor-version":[{"id":3755,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/432\/revisions\/3755"}],"wp:attachment":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/media?parent=432"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}