![\"\"](\"https:\/\/i0.wp.com\/pgtb.fr\/wp-content\/uploads\/2022\/02\/rnaseqoverview2.png?resize=750%2C505&ssl=1\")
<\/figure>\n<\/div>\n\n\n<\/p>\n\n\n\n
Services<\/p>\n\n\n\n
The number of samples to be determined using the PGTB depends on the sequencer, the flow cell and the target number of reads. It is recommended that replicates be included for RNA-seq analyses.<\/p>\n\n\n\n
Sequencing can be performed using short reads on the Illumina NextSeq 2000 or using long reads on the Oxford Nanopore Technologies P2 Solo.<\/p>\n\n\n\n
<\/p>\n\n\n\n
Requirements<\/p>\n\n\n\n
Illumina sequencing: 1 \u00b5g of total RNA and RIN >7 <\/p>\n\n\n\n
ONT sequencing: Three kits are available, depending on your requirements and the resources available:<\/p>\n\n\n\n
- \n
- Sequencing of native full-length mRNA strands (>500 ng of mRNA)<\/li>\n<\/ul>\n\n\n\n
- \n
- Stranded sequencing of unamplified full-length cDNA (>100 ng mRNA)<\/li>\n<\/ul>\n\n\n\n
- \n
- Stranded sequencing of amplified full-length cDNA (>10 ng of mRNA)<\/li>\n<\/ul>\n\n\n\n
Results<\/p>\n\n\n\n
Sent by email or uploaded to a server<\/p>\n\n\n\n
Depending on the service requested:<\/p>\n\n\n\n
- \n
- Demultiplexed FASTQ files<\/li>\n\n\n\n
- Sequencing run report (MultiQC <\/a>forIllumina, PycoQC <\/a>forOxford Nanopore)<\/li>\n\n\n\n
- Analyse sur DRAGEN RNA pipeline<\/a> pour le s\u00e9quen\u00e7age Illumina<\/li>\n<\/ul>\n\n\n\n
Associated publications<\/p>\n\n\n\n
Lopes, M., Vincent, A., Thomas, F., Clouard, C., Comte, R., Brien, M., Chambeaud, J., H\u00e9rault, F., Guichoux, E., Boury, C<\/strong>., Resmond, R., & Merlot, E., 2024. Data paper: Dataset describing the effects of environmental enrichment and sows\u2019 characteristics on the peripheral blood mononuclear cell transcriptome. Animal \u2013 Open Space<\/em>.https:\/\/doi.org\/10.1016\/j.anopes.2024.100078<\/a><\/p>","protected":false},"excerpt":{"rendered":"
La transcriptomique consiste \u00e0 s\u00e9quencer tout ou partie des ARN transcrits (ARNm, miRNA, ARN non-codants etc…) dans le g\u00e9nome \u00e0 un moment donn\u00e9 et dans des conditions donn\u00e9es. Cette technique utilise le s\u00e9quen\u00e7age haut d\u00e9bit pour identifier et quantifier le transcriptome. Ces analyses permettent d’\u00e9tudier l’expression de g\u00e8nes, les r\u00e9seaux d’expression des g\u00e8nes et lesContinue reading \u00ab\u00a0Transcriptomique\u00a0\u00bb<\/span><\/a><\/p>","protected":false},"author":216247060,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"jetpack_seo_schema_type":"","_wpcom_ai_launchpad_about_page":false,"_wpcom_ai_launchpad_gallery_page":false,"_wpcom_ai_launchpad_contact_page":false,"_wpcom_ai_launchpad_events_page":false,"_wpcom_ai_launchpad_video_page":false,"_wpcom_ai_launchpad_portfolio_piece":false,"footnotes":""},"class_list":["post-425","page","type-page","status-publish","hentry","entry"],"jetpack_likes_enabled":true,"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/PdFmHm-6R","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/425","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/users\/216247060"}],"replies":[{"embeddable":true,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/comments?post=425"}],"version-history":[{"count":27,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/425\/revisions"}],"predecessor-version":[{"id":4283,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/425\/revisions\/4283"}],"wp:attachment":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/media?parent=425"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
- Analyse sur DRAGEN RNA pipeline<\/a> pour le s\u00e9quen\u00e7age Illumina<\/li>\n<\/ul>\n\n\n\n
- Stranded sequencing of amplified full-length cDNA (>10 ng of mRNA)<\/li>\n<\/ul>\n\n\n\n
- Stranded sequencing of unamplified full-length cDNA (>100 ng mRNA)<\/li>\n<\/ul>\n\n\n\n