{"id":3757,"date":"2026-01-08T10:23:35","date_gmt":"2026-01-08T09:23:35","guid":{"rendered":"https:\/\/pgtb.fr\/?page_id=3757"},"modified":"2026-05-05T13:58:09","modified_gmt":"2026-05-05T11:58:09","slug":"genotypage-de-snp-par-sequencage-snpseq","status":"publish","type":"page","link":"https:\/\/pgtb.fr\/en\/genotypage-de-snp-par-sequencage-snpseq\/","title":{"rendered":"SNP genotyping by sequencing (SNPseq)"},"content":{"rendered":"<p class=\"wp-block-paragraph translation-block\">The PGTB offers <strong>a method for SNP genotyping using high-throughput sequencing<\/strong> which has several advantages:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u2013 the ability to analyse up to 192 markers in a single multiplex<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u2013 the ability to genotype between 95 and 1,140 samples in a single run <\/p>\n\n\n\n<p class=\"wp-block-paragraph\">- the identification of all types of polymorphisms present in the region surrounding the SNP of interest.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Services<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">We offer a full service comprising:<\/p>\n\n\n\n<ul id=\"Le-s\u00e9quenceur-ISeq-100\" class=\"wp-block-list\">\n<li>the design of multiplex primers based on the SNP marker sequence file<\/li>\n\n\n\n<li>their amplification using highly multiplexed PCR (up to 192 markers tested)<\/li>\n\n\n\n<li>the construction of libraries and Illumina sequencing (up to 1,140 individuals per run, including 95 repeat samples for data validation during the initial analysis used to develop the markers)<\/li>\n\n\n\n<li>the bioinformatics analysis used to generate the genotype table.<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">These steps can be adapted to suit specific requirements (number of individuals and markers to be analysed, etc.).<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Requirements<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/pgtb.fr\/wp-content\/uploads\/2026\/05\/I-Prerequis-pour-le-genotypage-de-SSR-et-SNP-1.pdf\" data-type=\"link\" data-id=\"https:\/\/pgtb.fr\/wp-content\/uploads\/2026\/05\/I-Prerequis-pour-le-genotypage-de-SSR-et-SNP-1.pdf\">Requirements for SNPseq<\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\">Results<\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Sent by email or uploaded to a server.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">The sequencing FASTQ files and the analysis results sent in Excel format, including:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>the coverage of each locus<\/li>\n\n\n\n<li>allelic information<\/li>\n\n\n\n<li>the genotype table<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>","protected":false},"excerpt":{"rendered":"<p>La PGTB propose une m\u00e9thode de g\u00e9notypage de SNP par s\u00e9quen\u00e7age haut-d\u00e9bit qui pr\u00e9sente plusieurs avantages&nbsp;: &#8211; la possibilit\u00e9 de passer jusqu&rsquo;\u00e0 192 marqueurs dans un seul multiplex &#8211; la possibilit\u00e9 de g\u00e9notyper de 95 \u00e0 1140 \u00e9chantillons en une fois -la r\u00e9v\u00e9lation de l&rsquo;ensemble des types de polymorphismes pr\u00e9sents dans la zone environnante du<a class=\"more-link\" href=\"https:\/\/pgtb.fr\/en\/genotypage-de-snp-par-sequencage-snpseq\/\">Continue reading <span class=\"screen-reader-text\">\u00ab\u00a0G\u00e9notypage de SNP par s\u00e9quen\u00e7age (SNPseq)\u00a0\u00bb<\/span><\/a><\/p>","protected":false},"author":216247060,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"advanced_seo_description":"","jetpack_seo_html_title":"","jetpack_seo_noindex":false,"jetpack_seo_schema_type":"","_wpcom_ai_launchpad_about_page":false,"_wpcom_ai_launchpad_gallery_page":false,"_wpcom_ai_launchpad_contact_page":false,"_wpcom_ai_launchpad_events_page":false,"_wpcom_ai_launchpad_video_page":false,"_wpcom_ai_launchpad_portfolio_piece":false,"footnotes":""},"class_list":["post-3757","page","type-page","status-publish","hentry","entry"],"jetpack_likes_enabled":true,"jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/PdFmHm-YB","jetpack-related-posts":[],"_links":{"self":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/3757","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/users\/216247060"}],"replies":[{"embeddable":true,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/comments?post=3757"}],"version-history":[{"count":12,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/3757\/revisions"}],"predecessor-version":[{"id":4232,"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/pages\/3757\/revisions\/4232"}],"wp:attachment":[{"href":"https:\/\/pgtb.fr\/en\/wp-json\/wp\/v2\/media?parent=3757"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}